Scientists Develop DNA- and Marker-free Genome-editing System using Zygotes in RiceApril 17, 2019
The introduction of CRISPR-Cas9 system has led to the development of rapid and cost-effective procedures to generate new mutant populations in plants. Even if genome-edited plants from several species have been developed successfully using a technique wherein a Cas9-guide RNA (gRNA) expression cassette and selectable marker are integrated into the genomic DNA through Agrobacterium tumefaciens-mediated transformation or particle bombardment, CRISPR-Cas9 system increases the chance for off-target modifications, and foreign DNA sequences cause legislative concerns about GMOs. Thus, DNA-free genome editing has been developed, which involves the transport of preassembled Cas9-gRNA ribonucleoproteins (RNPs) into protoplasts derived from somatic tissues by polyethylene glycol-calcium mediated transfection in tobacco, Arabidopsis, rice, potato, etc. or into embryo cells thru the use of a gene gun in maize and wheat.
Experts from RIKEN Cluster for Science, Technology and Innovation Hub reported in Nature Plants a genome-editing system via direct delivery of Cas9–gRNA RNPs into plant zygotes. Cas9–gRNA RNPs were integrated into rice zygotes produced by in vitro fertilization of isolated gametes and the zygotes were cultured into mature plants in the absence of selection agents, resulting in the regeneration of rice plants with targeted mutations in around 14 to 64 percent of plants.
Based on the study, the new plant genome-editing system has a huge potential for the continuous development of rice and other vital crops.
Read the abstract in Nature Plants.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Scientists Develop Methods to Validate Gene Regulation Networks
- Plants Use Genetic Backup to Protect Stem Cells
- Sex Determination of Papaya Trees Now Possible
- Chinese Scientists' Genome-Edited Wheat to Help Control Weeds
- Pakistan Announces National Coordinated Trials of 85 Bt Cotton Varieties
- China's GM Approval Process Could Cost Farmers Billions
- VIB CRISPR Maize Field Trial Granted Permit
- Vietnamese Scientists Characterize Rice Gene OsSWEET14
- Nematode Resistant Eggplant Developed Through Host-induced Silencing
- HB4 Wheat Compositionally Equivalent to Non-transgenic Wheat
Beyond Crop Biotech
- Tropical Grass Genetic Breakthrough to Help Develop Climate-Friendly Cattle Farms
- National University of Colombia Scientists Gene-Edited Smallest Tomato in the World
- Researchers Characterize Lignin of Rice Mutants Generated Using CRISPR-Cas9
- Scientists Develop DNA- and Marker-free Genome-editing System using Zygotes in Rice
Read the latest:
- Biotech Updates (November 29, 2023)
- Gene Editing Supplement (November 29, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: