Biotech Updates

Vietnamese Scientists Characterize Rice Gene OsSWEET14

April 17, 2019

Xanthomonas oryzae pv. oryzae (Xoo) causes serious bacterial leaf blight disease disease to many major rice cultivars, including the Bacthom 7 variety. OsSWEET14 belongs to group III of the OsSWEET family encoded sugar transport proteins and seem to be one of the susceptibility genes for infection of Xoo in rice. In a study published in the Journal of Agriculture and Rural Development, the pathotype of 18 Xoo strains collected from eight provinces in North of Vietnam with Bacthom 7 genotype was evaluated.

The findings showed that Bacthom 7 variety was very sensitive to 17/18 Xoo strains. The expression of Bacthom 7 OsSWEET14 was induced by three of five representative Xoo strains, including XO_52, XO_59 and XO_69. The DNA fragment of Bacthom 7 OsSWEET14 promoter was isolated, cloned into the pGEM-T vector and sequenced completely. The isolated promoter showed the similarity of more than 99 percent with the published OsSWEET14 promoter sequence (AP014967.1 and CP012619.1), contained four effector binding elements (EBE), including TalC, Tal5, PthXa3, and AvrXa7 that recognized by the type III-secretory transcription activator-like (TAL) proteins of the Xoo. This research is the basis for generating bacterial leaf blight disease resistant Bacthom 7 rice variety in Vietnam.

Read the original article (Vietnamese) in the Journal of Agriculture and Rural Development.