Nematode Resistant Eggplant Developed Through Host-induced SilencingApril 17, 2019
RNA interference (RNAi)-based host-induced gene silencing (HIGS) is one of the new tools used in fighting phytonematode infection in crop plants. Mi-msp-1, an effector gene expressed in the subventral pharyngeal gland cells of root-knot nematode (Meloidogyne incognita) has a vital function in the parasitic process.
Scientists from Indian Agricultural Research Institute and other institutions generated six eggplant transformants with a single copy RNAi transgene of Mi-msp-1. The transgene was expressed in T1, T2 and T3 transgenic lines for which a detrimental effect on root-knot nematodes penetration, development and reproduction was documented upon challenge infection with nematode juveniles. Furthermore, the post-parasitic nematode stages derived from the transgenic plants exhibited long-term RNAi effect as manifested in the targeted downregulation of Mi-msp-1.
The results of the study imply that HIGS of Mi-msp-1 improved resistance to nematode in eggplant and protect the plant against RKN parasitism at the very early stage.
For more details, read the results published in Transgenic Research.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Scientists Develop Methods to Validate Gene Regulation Networks
- Plants Use Genetic Backup to Protect Stem Cells
- Sex Determination of Papaya Trees Now Possible
- Chinese Scientists' Genome-Edited Wheat to Help Control Weeds
- Pakistan Announces National Coordinated Trials of 85 Bt Cotton Varieties
- China's GM Approval Process Could Cost Farmers Billions
- VIB CRISPR Maize Field Trial Granted Permit
- Vietnamese Scientists Characterize Rice Gene OsSWEET14
- Nematode Resistant Eggplant Developed Through Host-induced Silencing
- HB4 Wheat Compositionally Equivalent to Non-transgenic Wheat
Beyond Crop Biotech
- Tropical Grass Genetic Breakthrough to Help Develop Climate-Friendly Cattle Farms
- National University of Colombia Scientists Gene-Edited Smallest Tomato in the World
- Researchers Characterize Lignin of Rice Mutants Generated Using CRISPR-Cas9
- Scientists Develop DNA- and Marker-free Genome-editing System using Zygotes in Rice
Read the latest:
- Biotech Updates (May 31, 2023)
- Gene Editing Supplement (May 31, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: