CRISPR-Cas9 Reveals Role of SlPHO1;1 in Phosphate Nutrition of Tomato Seedlings
December 12, 2018
In vascular and nonvascular plants, PHOSPHATE1 (PHO1) homologs have vital functions in the harvesting and transferring of phosphate. In tomatoes, six genes (SlPHO1;1‐SlPHO1;6) are homologous to Arabidopsis' PHO1 (AtPHO1). Thus, scientists from Chinese Academy of Sciences used CRISPR-Cas9 to reveal the function of PHO1 in phosphate nutrition of tomatoes.
An analysis showed that SlPHO1 family is composed of three clusters, wherein SlPHO1;1 is the most similar to AtPHO1. SlPHO1;1 deletion mutants induced by CRISPR-Cas9 displayed typical symptoms of inorganic phosphate (Pi) starvation, such as decreased shoot fresh weight and increased root fresh weight, therefore having a greater root-to-shoot ratio. Mutants also exhibited higher levels of anthocyanin and soluble Pi in the root and less in the shoot.
The findings imply that indicate that SlPHO1;1 plays an important role in Pi transport in the tomato at the seedling stage.
Read the research article in Physiologia Plantarum for more information.
The Crop Biotech Update is a weekly newsletter of ISAAA, a not-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- Nobel Laureates: Fear of GM Crops Prevents Society from Benefiting from the Technology
- WRI Report Says GMOs and Gene Editing Can Help Improve Crop Breeding to Boost Yields to Feed the World
- Alliance for Science Ghana Calls for Passage of Plant Breeders' Bill into Law
- USDA to Release Final ‘Bioengineered' Food Labeling Standard
- Report Highlights USDA Technology Innovations
- Biotech Cotton in Pakistan Reached Highest Adoption in 2017
- OECD Study Provides New and Detailed Evidence on Market Concentration in Seed and GM Technology
- Aarhus University Researchers Release New Insights in Rust Resistance in Wheat
- Plants as Antifungal Factories
-
Research Highlights
- Transgenic Pm3e Wheat Show High Resistance to Powdery Mildew
- International Research Team Finds Novel Function of Plant Hormone Jasmonic Acid
-
Plant Breeding Innovations
- Researchers Discover Mechanism Disrupting CRISPR-Cas9
- CRISPR-Cas9 Reveals Role of SlPHO1;1 in Phosphate Nutrition of Tomato Seedlings
-
Announcements
- International Conference on Microbes for Sustainable Agriculture
-
Resources
- Updated ISAAA Infographics: Do you know where biotech crops are grown?
-
Read the latest: - Crop Biotech Update (October 5, 2022)
- Genome Editing Supplement (October 5, 2022)
- Gene Drive Supplement (September 28, 2022)
-
Subscribe to CBU: - Share
- Tweet