CRISPR Editing Tested on Carotenoid Synthesis Genes in TomatoMay 30, 2018
Carotenoids are plant secondary metabolites that are important in the diet of higher animals due to their functions in important physiological processes. The team of Caterina D'Ambrosio from Agenzia Lucana per lo Sviluppo e l'Innovazione in Agricoltura in Italy tested the efficacy of CRISPR-Cas9 in tomato by targeting two key genes of carotenoid biosynthesis, Psy1 and CrtR-b2.
Psy1 and CrtR-b2 were chosen as targets for CRISPR since their knockout is easily detectable. Two CRISPR-Cas9 constructs were designed to target each gene. Thirty-four out of forty-nine (69%) transformed plants showed the loss-of-function phenotypes due to the editing of both loci. However, by including plants edited only in either of the two loci, the genome-editing rate reached 84%.
These results prove that the CRISPR-Cas9 system can be an efficient and quick method for generating useful mutations in tomato for use in breeding programs.
For more information, read the article in Transgenic Research.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- FAO: Incorporating Biodiversity as a Priority in Agri Can Yield Multiple Benefits
- African Countries Need to Push Climate Smart Agriculture
- Researchers Engineer Cell-Like Structure for Photosynthesis
- Golden Rice Gets Approval from US FDA
- Special Screening of Documentary on GMOs held for Legislators in the Philippines
- EFSA Publishes Scientific Opinion on GM Maize 4114
- Gene from Taro Confers Aphid Resistance in Indian Mustard
- Endogenous Proteins Expression in Maize Tested in a Multi-Location Fields in India
- Candidate Gene for Drought Tolerance Found in Wheat
Beyond Crop Biotech
- New Arabica Coffee Genome Sequence Made Available
- Transgenic Pigs that Digest Grains Better can Reduce Pork Industry's Carbon Footprint
- Conversations on Climate Change
- Workshop on Marker Assisted Breeding in Oilseed Mustard
- Video: Uganda's GMO Potato Story
- Chinese Researchers Develop Herbicide Tolerant Watermelon
- Scientists Find Resistance Gene Against Rice Blast
- SDG8 Genes Involved in Floral Transition in Rapeseed
- CRISPR Editing Tested on Carotenoid Synthesis Genes in Tomato
Read the latest:
- Biotech Updates (September 27, 2023)
- Gene Editing Supplement (September 27, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: