Gene Promoter Used to Optimize CRISPR for Targeted Genome Editing in MaizeApril 4, 2018
The promoters driving both Cas9 and sgRNAs are quite important for efficient genome editing by CRISPR-Cas9 in plants. The team of Chao Feng from the Chinese Academy of Sciences tested the combination of maize dmc1 gene promoter and the U3 promoter for the Cas9 and sgRNA, respectively.
Three loci in the maize genome were selected for targeting. The T0 plants developed were efficiently edited at the target sites with mutants accounting for about 66%. The mutations in T0 plants were found to be stably transmitted to the T1 generation. Analysis also indicated that no off‐target mutations were detected at potential off-target sites.
These results show that the dmc1 promoter-controlled CRISPR-Cas9 system is highly efficient for maize. This study provides evidence that the optimization of the promoters for the CRISPR-Cas9 system can enhance the efficiency of targeted genome editing in plants.
For more information, read the article in Plant Biotechnology Journal.
The Crop Biotech Update is a weekly newsletter of ISAAA, a not-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Agribiotech Can Unlock Business Opportunities in Africa
- High Hopes as Uganda's Biotech Bill Gets 'Second Chance'
- Scientists Publish Additional Soybean Reference Genomes
- Australian Vine Helps Boost Soybean Yield
- CAS Researchers Discover Evidence of the Beginning Rice Cultivation
- USDA FAS-GAIN Reports Agri-biotech Updates in Pakistan
- Genome Archaeologists Uncover Origin of Plant Hormone Auxin
- The Royal Society Report Says UK Public Cautiously Optimistic about Genetic Technologies
- Sugar Transporters in Tea Plants Also Involved in Plant Response to Stresses
- GhPEPC2 Gene Regulates Seed Oil Accumulation in Cotton
- Researchers Find Gene Regulating Plant Growth and Pest Resistance in Rice
Plant Breeding Innovations
- Researchers Study Morphological Impact of ERECTA Genes in Rice Using CRISPR
- Gene Promoter Used to Optimize CRISPR for Targeted Genome Editing in Maize
- Chinese Scientists Perform Gene Replacement in Rice
- US Agriculture Secretary Issues USDA Statement on Plant Breeding Innovation
Beyond Crop Biotech
- Engineering Yeast to Make Non-Narcotic Cough Suppressant
- Animal Scientist Highlights Role of Genetic Modification in Livestock Health, Growth and Well-being
- 2018 IPBO Conference
Read the latest:
- Crop Biotech Update (November 23, 2022)
- Genome Editing Supplement (November 23, 2022)
- Gene Drive Supplement (October 26, 2022)
Subscribe to CBU: