Researchers Test CRISPR-Cas9 Gene Editing on Brown PlanthopperFebruary 21, 2018
The brown planthopper (Nilaparvata lugens), or BPH, is one of the most destructive insect pests in Asia, demonstrating high fertility and causing huge crop losses in rice yield. However, genomic studies on BPH are seriously hampered by lack of genetic tools. The team of Wen-Hua Xue from Zhejiang University in China tried to apply CRISPR-Cas9 on BPH by targeting two eye pigmentation genes to generate mutations.
The team found that injection of a single guide RNA targeting the cinnabar gene (Nl-cn) into BPH eggs induced mutations in the founder generation (G0). These mutations were then inherited by the following generation, leading to bright red compound eyes and ocelli. Another gene, BPH white gene (Nl-w), was also targeted by a separate CRISPR sgRNA. This generated a high mutant rate of up to 27.3%, resulting in mosaic eyes consisting of white and lightly pigmented ommatidia in both G0 and G1individuals.
These results show that CRISPR-Cas9 gene editing can be done on a hemipteran insect, offering a valuable tool for pest management of BPH.
For more information, read the article in Insect Biochemistry and Molecular Biology.
The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- TWAS Elects New Fellows for 2018
- FAO and OECD Promote Responsible Investment in Agri
- Social Media Campaign Empowers Women in Science
- Meta-Analysis of 21 Years of Data Reveals Benefits of GE Maize
- Research Team Finds Gene that Improves Plant Growth and Conversion to Biofuels
- Green Super Rice for A Greener Revolution
- Study Reveals How Plants Get Their Nitrogen Fix
- Sainsbury Laboratory Scientists Solved 79-Year-Old Mystery of Plant Response to Heat
- International Research Team Gains New Insights into Tomato Breeding
- Mustard Gene Improves Health-Promoting Compounds in Tomato
- Small Signaling Peptide Enhances Drought Tolerance in Rice
- ShCIGT Confers Abiotic Stress Tolerance in Tomato
Plant Breeding Innovations
- ZFN Used to Study Rice SSIVa Gene
- Researchers Test CRISPR-Cas9 Gene Editing on Brown Planthopper
- Disruption of OsSEC3A Induces Plant Defense Responses in Rice
Beyond Crop Biotech
- Gene that Could Make More Viable Ever-bearing Strawberries
- Scientists Use CRISPR to Make Cellular Recorders
- International Biotechnology and Research Conference 2018
Subscribe to CBU: