ZFN Used to Study Rice SSIVa Gene
February 21, 2018| |
One of the popular genome editing technologies used in plants is the Zinc Finger Nuclease (ZFN), which are proteins composed of a zinc finger-based DNA binding domain and a DNA cleavage domain. The team of Yu-Jin Jung of the Hankyong National University in South Korea used ZFNs to target SSIVa, a soluble starch synthase involved in starch biosynthesis pathway.
The team used ZFN-mediated targeted gene disruption on the SSIVa rice gene in order to study the role of the gene. Analysis of the edited plants revealed the absence of SSIVa mRNAs as well as low starch contents and dwarf phenotypes. Interestingly, the analysis also showed that SSIVa had no effect on other starch synthesis related genes since their expressions remained the same with the wildtype levels.
Therefore, the engineered ZFNs induced mutations at SSIVa locus in rice resulting in changes in plant height and starch content.
For more on this study, read the article in Biotechnology and Bioprocess Engineering.
| |
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- TWAS Elects New Fellows for 2018
- FAO and OECD Promote Responsible Investment in Agri
- Social Media Campaign Empowers Women in Science
- Meta-Analysis of 21 Years of Data Reveals Benefits of GE Maize
- Research Team Finds Gene that Improves Plant Growth and Conversion to Biofuels
- Green Super Rice for A Greener Revolution
- Study Reveals How Plants Get Their Nitrogen Fix
- Sainsbury Laboratory Scientists Solved 79-Year-Old Mystery of Plant Response to Heat
- International Research Team Gains New Insights into Tomato Breeding
-
Research Highlights
- Mustard Gene Improves Health-Promoting Compounds in Tomato
- Small Signaling Peptide Enhances Drought Tolerance in Rice
- ShCIGT Confers Abiotic Stress Tolerance in Tomato
-
Beyond Crop Biotech
- Gene that Could Make More Viable Ever-bearing Strawberries
- Scientists Use CRISPR to Make Cellular Recorders
-
Announcements
- International Biotechnology and Research Conference 2018
-
Plant
- ZFN Used to Study Rice SSIVa Gene
- Researchers Test CRISPR-Cas9 Gene Editing on Brown Planthopper
- Disruption of OsSEC3A Induces Plant Defense Responses in Rice
-
Read the latest: - Biotech Updates (April 17, 2024)
- Gene Editing Supplement (April 10, 2024)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet