Plant-produced Zika Virus Vaccine Confers Protective Immunity in MiceAugust 30, 2017
The global Zika virus (ZIKV) outbreak and its link to fetal and newborn microcephaly and severe neurological complications in adults call for the urgent development of ZIKV vaccines. Arizona State University researchers, led by Ming Yang, developed a subunit vaccine based on the ZIKV envelope (E) protein and investigated its immunogenicity in mice.
Expression of ZIKV E (zE) in tobacco (Nicotiana benthamiana) resulted in rapid accumulation in plant leaves. Biochemical analysis revealed that plant-produced ZIKV E (PzE) exhibited specific binding to a panel of monoclonal antibodies that recognize various zE conformational epitopes. Furthermore, the PzE can be purified to >90% homogeneity with a one-step chromatography process.
Two doses of PzE drew out both potent zE-specific antibody and cellular immune responses in mice. Most importantly, the concentration of zE-specific and neutralizing antibodies exceeded the threshold and correlates with protective immunity against multiple strains of ZIKV.
These results demonstrate the feasibility of plant-produced ZIKV protein antigen as effective, safe and affordable vaccines against ZIKV.
For more on this study, read the article in Plant Biotechnology Journal.
The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- International Research Team Maps Jumping Genes of Maize
- Nigeria Should Turn to Biotech Amidst Population Boom
- Delays in Biotech Crop Approvals in Africa Amplifies Malnutrition
- Genetic Map Reveals Heat Tolerance Traits in Peas
- Plant 'Smells' Insect Foe, Defends Self
- MSU Food Literacy Poll Shows Consumers Are Confused about GM Foods
- Study Says Americans OK with GM for Health Care, Still Wary About Food
- ABCA Releases Updated Agricultural Biotechnology Guide
- Biotech Crop Regulation Based on Sound Science Needed for Myanmar, says Permanent Secretary
- Study Finds Hormonal Interactions Regulate Growth of Plant Roots
- GE Plants with Algae Genes Could Improve Crop Yields
- GaMYB85 Plays a Role in Drought Tolerance in Transgenic Arabidopsis
Plant Breeding Innovations
- CRISPR-mediated Knockout of SaF/SaM Overcomes the Hybrid Male Sterility in Rice
Beyond Crop Biotech
- Biotech Moths to be Field Tested in NY
- Plant-produced Zika Virus Vaccine Confers Protective Immunity in Mice
Subscribe to CBU: