GaMYB85 Plays a Role in Drought Tolerance in Transgenic ArabidopsisAugust 30, 2017
MYB transcription factors (TFs) are involved in diverse biological, functional, and structural processes. Previously, very few studies on R2R3 MYB have been conducted in cotton in response to abiotic stresses. Hamama Islam Butt from the Institute of Cotton Research of Chinese Academy of Agricultural Science expressed GaMYB85, a cotton R2R3 MYB TF, in Arabidopsis thaliana to study the function of the TF in the transgenic plants.
Arabidopsis plants overexpressing GaMYB85 exhibited a higher seed germination rate in response to mannitol and salt stresses, and higher drought avoidance efficiency than wild-type plants. These plants also had notably higher levels of free proline and chlorophyll with subsequent lower water loss rates and higher relative water content.
Germination of GaMYB85 transgenics was found to be more sensitive to abscisic acid (ABA). Moreover, when subjected to treatment with different concentrations of ABA, transgenic plants showed reduced stomatal density, with greater stomatal size and lower stomatal opening rates than wild-type plants. Expression of GaMYB85 led to enhanced expression of stress-related marker genes such as RD22, ADH1, RD29A, P5CS, and ABI5.
These results show previously unknown roles of GaMYB85. This study found that it confers good drought, salt, and freezing tolerance, via an ABA-induced pathway. This TF can potentially be used to develop improved abiotic stress tolerance in cotton.
For more information, read the article in BMC Plant Biology.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- International Research Team Maps Jumping Genes of Maize
- Nigeria Should Turn to Biotech Amidst Population Boom
- Delays in Biotech Crop Approvals in Africa Amplifies Malnutrition
- Genetic Map Reveals Heat Tolerance Traits in Peas
- Plant 'Smells' Insect Foe, Defends Self
- MSU Food Literacy Poll Shows Consumers Are Confused about GM Foods
- Study Says Americans OK with GM for Health Care, Still Wary About Food
- ABCA Releases Updated Agricultural Biotechnology Guide
- Biotech Crop Regulation Based on Sound Science Needed for Myanmar, says Permanent Secretary
- Study Finds Hormonal Interactions Regulate Growth of Plant Roots
- GE Plants with Algae Genes Could Improve Crop Yields
- GaMYB85 Plays a Role in Drought Tolerance in Transgenic Arabidopsis
Beyond Crop Biotech
- Biotech Moths to be Field Tested in NY
- Plant-produced Zika Virus Vaccine Confers Protective Immunity in Mice
- CRISPR-mediated Knockout of SaF/SaM Overcomes the Hybrid Male Sterility in Rice
Read the latest:
- Biotech Updates (May 31, 2023)
- Gene Editing Supplement (May 31, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: