CRISPR-Cas9 Genome Editing in RapeseedAugust 23, 2017
CRISPR-Cas9 is a valuable tool for research and has been widely applied to different plant species. However, the efficiency of this method is not available for the allotetraploid rapeseed (Brassica napus), an important oilseed crop. Huazhong Agricultural University researchers evaluated the mutation efficiency of the CRISPR-Cas9 for 12 genes in rapeseed, including paralogous genes.
CRISPR efficiently induced targeted mutations both for single locus and multiple loci targeting in rapeseed. CRISPR also induced mutations in paralogous genes, or different genes with the same function in a species. Since the paralogous genes in rapeseed have highly similar sequences, they were successfully targeted using only a single sgRNA. No off-target mutations were identified in the CRISPR-Cas9 lines, indicating that the mutations induced were highly specific.
Half of the mutations, including homozygotes, bi-alleles, and heterozygotes were also stably inherited in the next generation (T1) without any new mutations or reversions.These results demonstrate that CRISPR-Cas9 can be an efficient tool for targeted genome modifications at multiple loci in rapeseed.
For more information, read the article in Scientific Reports.
The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Terminology Matters in Biosafety Communication
- Biologists Show How Plants Turn Off Genes They Don't Need
- Researchers Describe Gene that Makes Large, Plump Tomatoes
- Australian OGTR Receives License Application for Commercial Release of GM Cotton
- Vietnam Will Have to Continue Importing Maize
- Davao Stakeholders Updated on Crop Biotech and Philippine Biosafety Guidelines
- Green Revolution Genes Promise More Yield
- GmSN1 Overexpression Enhances Virus Resistance in Arabidopsis and Soybean
- Role of PtrZPT2-1 Gene in Plant Tolerance to Multiple Abiotic Stresses
Plant Breeding Innovations
- Targeted Mutagenesis in Tetraploid Switchgrass
- CRISPR-Cas9 Genome Editing in Rapeseed
Beyond Crop Biotech
- Plant-produced African Horse Sickness Virus Novel Vaccine
From the BICs
- DA-Davao Info Officers Explore Social Media for Sharing Agri Innovations
- PARC Adopts New Techniques for Agricultural Development in Pakistan
- Updated Pocket Ks on Biotech Now Available
Subscribe to CBU: