RPW8.1 Gene Boosts Pattern-Triggered Immunity against Multiple Pathogens
August 2, 2017| |
The Arabidopsis gene RESISTANCE TO POWDERY MILDEW8.1 (RPW8.1) confers resistance to virulent fungal pathogens. However, the underlying mechanism remains unclear. Yan Li and a team of researchers from Sichuan Agricultural University studied the gene by ectopic expression of RPW8.1 in Arabidopsis and rice. This boosted pattern-triggered immunity (PTI), resulting in enhanced resistance against different pathogens in both crops.
In Arabidopsis, analysis revealed that ectopic expression of RPW8.1-YFP upregulated expression of many pathogen-associated molecular pattern (PAMP-)-inducible genes. The transgenic line exhibited pronounced PTI responses such as callose deposition, production of reactive oxygen species, expression of defense-related genes and hypersensitive response such as cell death.
Hence, the growth of a virulent bacterial pathogen was significantly inhibited in transgenic lines expressing RPW8.1-YFP. In rice, expression of RPW8.1-YFP led to enhanced resistance to the blast fungus Pyricularia oryzae and the bacterial pathogen Xanthomonas oryzae pv. oryzae.
These results suggest a connection between RPW8.1 function and PTI, and demonstrate the potential of RPW8.1 as a transgene for engineering disease resistance across crops.
For more information, read the article in Plant Biotechnology Journal.
| |
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- Methodological Flaws Found in Studies Reporting Adverse Effects of GM Crops
- Ghana Gov't Supports GE Research to Combat Armyworm
- New Assembly for Bread Wheat Genome has 10x Higher Contiguity
- USDA APHIS Announces Extension of Deregulation to GE Canola
- PABIC and ICCBS Hold ISAAA Report Launch
- CSIRO Decodes Genome of Megapests
- Pangasinan Stakeholders Learn about Bt Brinjal, Affirm Support for Bt Talong
- Study Reports SmartStax Maize Does Not Affect Non-Target Organisms
-
Research Highlights
- RPW8.1 Gene Boosts Pattern-Triggered Immunity against Multiple Pathogens
- Overexpression of Mutated ZmDA1 or ZmDAR1 Gene Improves Maize Yield via Enhanced Starch Synthesis
-
Beyond Crop Biotech
- Japanese Scientists Genetically Engineer A True Blue Chrysanthemum
-
From the BICs
- Pakistan and China to Work Together in Advancing Agriculture
- Challenges in Commercializing Biotech Crops Tackled in Seminar
-
Announcements
- European Biotech Week 2017
-
Plant
- Transcriptional Activation via CRISPR-dCas9 Mimics Overexpression Phenotypes in Arabidopsis
-
Read the latest: - Biotech Updates (April 17, 2024)
- Gene Editing Supplement (April 10, 2024)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet