CRISPR-Cas9-Mediated Deletion of a Target Gene in Indica RiceJune 14, 2017
CRISPR-Cas9 has been widely used to produce gene-specific edits in both mammalian and plant systems. Most modifications in plants have been small insertions or deletions, with only a few large target gene deletions, especially for indica rice.
The team of Ying Wang from Syngenta Biotechnology China designed several CRISPR sgRNAs and successfully deleted DNA fragments in the DENSE AND ERECT PANICLE 1 (DEP1) gene in the elite Indica rice line IR58025B. The team achieved up to 2% mutation frequency for a 430 bp target and a 9% frequency for the 10 kb target among T0 events.
Improvements in yield-related traits, such as dense and erect panicles and reduced plant height, were observed in dep1 homozygous T0 mutant plants produced via CRISPR-Cas9.
For more on this study, read the article in Plant Cell Reports.
The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Breakthrough Research Makes Synthetic Photosynthesis Possible
- Detailed New Maize Reference Genome Shows Its Deep Resources for Continued Adaptation
- Brazil Approves GM Sugarcane for Commercial Use
- Researchers Identify Gene Against Wheat Streak Mosaic Virus
- Study Shows Rice First Cultivated in China 10,000 Years Ago
- Filipino Crop Scientists Tackle Ways to Face Climate Change and Globalization
- South Korean Consumer Group Hosts ISAAA Brief 52 Seminar
- Scientists Discover that Symbiotic Fungi Get Carbon from Plants in the Form of Fatty Acids
- Apple Genome Sequence Published
- Alcohol dehydrogenase 1 Confers Abiotic and Biotic Stress Resistance in Arabidopsis
- Non-target Arthropod Risk Assessment for Bt Rice in China
Plant Breeding Innovations
- Functional Characterization of OsSAPK2 Using CRISPR-Cas9
- CRISPR-Cas9-Mediated Deletion of a Target Gene in Indica Rice
Beyond Crop Biotech
- Researchers Identify a Carotenoid Biosynthetic Gene in Rhodosporidium toruloides
- EFSA Releases Literature Review of Baseline Information on Assessment of RNAi-based GM Plants
Subscribe to CBU: