Biotech Updates

Overexpression of Del Gene from Snapdragon Improves Anthocyanin Accumulation and Abiotic Stress Tolerance in Tobacco

March 29, 2017

The co-expression of Rosea1 (Ros1) and Delila (Del) genes control the anthocyanin accumulation in snapdragon flowers. In tomato, their overexpression strongly induces anthocyanin accumulation. Korean researchers led by Aung Htay Naing from Kyungpook National University investigated how Del expression alone influences anthocyanin accumulation.

In tobacco, Del expression enhanced leaf and flower anthocyanin production through the regulation of NtCHS, NtCHI, NtF3H, NtDFR, and NtANS transcript levels. Transgenic overexpression lines displayed different anthocyanin colors, resulting from varying levels of biosynthetic gene transcripts. Under salt stress, the T2 generation had higher total polyphenol content, radical scavenging activities, antioxidant-related gene expression, as well as overall greater salt and drought tolerance than wildtypes.

These results suggest that Del overexpression elevates transcript levels of anthocyanin biosynthetic and antioxidant-related genes, leading to enhanced anthocyanin production and antioxidant activity, leading to improved abiotic stress tolerance.

For more information, read the article in BMC Plant Biology.