Reassessment of the Yield-Related Genes in Rice through CRISPRDecember 14, 2016
The CRISPR/Cas9 system has been successfully used as efficient tool for genome editing in a variety of species. Researchers from the Chinese Academy of Sciences in China aimed to use the CRISPR/Cas9 system to mutate genes reported to function as regulators of grain number (Gn1a), panicle architecture (DEP1), grain size (GS3), and plant architecture (IPA1) in rice.
Analysis of the first generation of transformed plants (T0) showed that the CRISPR/Cas9 system was highly efficient in inducing targeted gene editing, with the desired genes being edited in 42.5% (Gn1a), 67.5% (DEP1), 57.5% (GS3), and 27.5% (IPA1) of the transformed plants.
The T2 generation of the gn1a, dep1, and gs3 mutants exhibited enhanced grain number, dense erect panicles, and larger grain size, respectively. Semi-dwarf and grains with long awns were also observed in dep1 and gs3 mutants, respectively. However, ipa1 mutants showed two contrasting phenotypes, having either fewer tillers or more tillers, depending on the changes induced in the target region.
These data proves that multiple regulators of important traits can be modified in a single cultivar via CRISPR/Cas9 system, and aid in the study of complex regulatory networks and stacking of important traits in cultivated varieties.
For more on this promising study, read the full article in Frontiers in Plant Science.
The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- New CAST Paper Examines Trade and Biotech Issues
- Nigeria Academy of Science Declares Support for Biotech
- Water-saving Plants Help Develop Drought Resistant Crops
- To Fight World Hunger, Researchers Use Nuclear Methods to Study Pest Resistance in Corn
- Biotech Crops Contribute ~US$127,000M to Argentina's Economy
- Australia's OGTR Authorizes Commercial Release of GM Cotton
- Study Reveals Plants Learn New Habits, Too
- EFSA Shares Raw Data From Risk Assessment of Glyphosate
- USDA FAS-GAIN Releases Agri-biotech Updates in Portugal
- Tomato ERFs Found Vital for Resistance to Botrytis cinerea
Plant Breeding Innovations
- Reassessment of the Yield-Related Genes in Rice through CRISPR
Beyond Crop Biotech
- Generation of Gene-Edited Birds Using Sperm Transfection Assisted Gene Editing
- ISAAA Blog Post: The Trial of Bt Talong Field Trials
- The Science of GMOs
- Pocket K No. 53: Anti-allergy Biotech Crops
Subscribe to CBU: