Researchers Produce Fatty Alcohols from Yeasts
November 3, 2016https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-016-0647-2
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Oleaginous yeasts have been studied for lipid production. However, yeasts store lipids intracellularly, making recovery difficult and expensive. Therefore, National Renewable Energy Laboratory researchers led by Wei Wang investigate fatty acid-derived products which can be easily recovered and upgraded to fuels. The team aimed to produce fatty alcohols from Yarrowia lipolytica and Lipomyces starkeyi, by expressing the fatty acyl-CoA reductase gene from Marinobactor aquaeolei.
The team was able to produce 167 and 770 mg/L of fatty alcohols in shake flask from Y. lipolytica and L. starkeyi, respectively. When using a dodecane overlay during fermentation, almost all total fatty alcohols produced by Y. lipolytica and L. starkeyi were extracted into the dodecane phase. In both yeasts, long chain length and saturated fatty alcohols were predominant.
This study proves that oleaginous yeasts can serve as platforms for the production of fatty acid-derived biofuels.
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