Zinc Finger Nuclease System Developed for Targeted Genome Engineering in TomatoSeptember 28, 2016
Precise gene targeting in crops has been long-sought for studying gene function. A team of researchers, led by Zoe Hilioti of the Centre for Research and Technology Hellas in Greece developed a zinc finger nuclease (ZFN)-based technology for tomato (Solanum lycopersicum) and evaluated its efficiency in targeted gene mutagenesis.
The team designed a ZFN for the developmental regulator LEAFY-COTYLEDON1-LIKE4 (L1L4) and transiently expressed it in tomato seeds. The ZFN cleaved the target site and stimulated imperfect repair driven by non-homologous end-joining, resulting in the introduction of mutations into the target site. The mutations resulted in L1L4 mutations which conferred heterochronic phenotypes.
These results highlight the feasibility of a custom-designed ZFN in targeted mutagenesis of tomato, which may accelerate the crop's research and breeding.
For more information, read the article in Plant Cell Reports.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- U.S. Approves Arctic Fuji Apple
- Researchers Discover Non-Bt Protein for Corn Rootworm Control
- U.S. Government Updates Federal Regulatory Policy for Biotechnology
- Australian OGTR Invites Comments for GM Potato Field Trial
- Genes to Fight Powdery Mildew in Barley Identified
- Scientists Discover a Protein that Can Help Plants Tolerate Saline Conditions
- GM Maize Harvested in Vietnam Field Trial Sites
- OsABCG26 Vital in Anther Cuticle and Pollen Exine Formation in Rice
- Increasing Palmitic Acid Levels in Cotton Seed Oil thru Downregulation of GhKAS2
Beyond Crop Biotech
- Researchers Discover Gene that Can Reduce Female Mosquitoes
- Downregulation of GIGANTEA-like Genes Enhances Growth and Salt Tolerance in Poplar
- Bt Eggplant Resources
- More Updated Pocket Ks on Biotech Now Available
- Zinc Finger Nuclease System Developed for Targeted Genome Engineering in Tomato
- ISU Scientists Review Status of Genome-Edited Crops Regulation
Read the latest:
- Biotech Updates (February 1, 2023)
- Genome Editing Supplement (January 18, 2023)
- Gene Drive Supplement (January 25, 2023)
Subscribe to BU: