
A Rapid, Low-cost Protoplast Transfection System for Switchgrass
August 3, 2016 |
There has been resurgence in the use of protoplast systems for rapid screening of gene silencing and genome editing targets for siRNA, miRNA, and CRISPR technologies. For switchgrass (Panicum virgatum L.), it is necessary to develop plants with decreased cell wall recalcitrance to reduce processing costs in biofuel production. Hence, transgenic plants have been generated with altered cell wall chemistry, but with limited success.
Due to the cost, time, and effort required to screen transgenic plants, a protoplast system that can provide data at an early stage has potential to eliminate low performing candidate genes/targets prior to development of transgenic plants. However, protoplast isolation in switchgrass has proven costly, requiring expensive enzymes and high DNA quantities.
University of Tennessee researchers, led by Kellie P. Burris, reported a low-cost protoplast isolation system using a mesophyll culture approach and a cell suspension culture. Results from this work showed a significant cost reduction compared to previous methods of protoplast isolation in switchgrass. Furthermore, the efficiency of transformation was optimized despite a significant reduction in DNA quantity.
The method developed in this study paves the way for more efficient and cost-effective development of transgenic plants.
For more on this promising system, read the article in Plant Cell Reports.
|
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- Scientists Test Efficacy of Maize Resistant to African Maize Stem Borer in Kenya Highlands
- Cost of Developing a GM Crop Demystified
- Repair System Found in Algae to Yield New Biotechnology Tools
- Obama Signs GM Food Labeling Law
- Davao Media Gets Enlightened on Biotechnology
- 'Superstar' Rice Varieties Reduce Fertilizer Loss, Cut Down Pollution
- Researchers Identify Gene Controlling Waterlogging Tolerance in Barley
- UP Law Professors Point out Need for Biotech Communicators
- Scientists Identify Mechanism of Tomato Softening
- Breakthrough in Understanding Rubisco to Help Feed the World
-
Research Highlights
- Stomatal Carpenter 1 Regulates Stomata Patterning in Plants
- Researchers Identify Two Powdery Mildew Resistance Genes from Wild Chinese Grapevine
-
Beyond Crop Biotech
- A Rapid, Low-cost Protoplast Transfection System for Switchgrass
-
Resources
- The GMO Revolution
-
Plant
- Oligonucleotide-Mediated Genome Editing in Plants
-
Read the latest: - Biotech Updates (April 30, 2025)
- Gene Editing Supplement (April 30, 2025)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet