Ectopic Expression of PtCYP714A3 from Populus trichocarpa Improves Salt Tolerance in Transgenic RiceMarch 16, 2016
In Arabidopsis thaliana and Oryza sativa, the cytochrome P450 (CYP) 714 protein family represents a unique group of CYP monooxygenase, shoot-specific regulator in plant development through gibberellin deactivation. Chinese Academy of Sciences researchers, led by Cuiting Wang, characterized a homologue of rice and Arabidopsis CYP from Populus trichocarpa, PtCYP714A3.
PtCYP714A3 was found highly expressed in cambium–phloem tissues and was induced by salt stress in poplar. Ectopic expression of PtCYP714A3 in rice led to semi-dwarfed phenotype with enhanced tillering and reduced seed size. Transgenic lines with significant expression of PtCYP714A3 also accumulated lower GA level than wild-type (WT) plants with some GA biosynthesis genes significantly suppressed in the transgenic plants.
Transgenic rice plants also exhibited enhanced tolerance to salt and maintained more sodium in both shoot and root tissues under salinity stress. These results suggest a crucial role of PtCYP714A3 in shoot responses to salt toxicity in rice and provide basis for engineering of salt-tolerant crops.
To learn more on this study, read the full article on Plant Biotechnology Journal.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Suppressing Growth: Opposition to GMOs Hurting Developing Nations
- New Study Identifies Sweet Corn Genes for Crowding Tolerance
- Study Suggests Climate Change Impact on Agriculture is Underestimated
- Researchers Discover Gene That Protects against Toxic Byproducts of Photosynthesis
- Scientists Unravel Unknown Receptors and Mechanism for Plant Fertilization
- New EU Project Focuses on Crops Important for Food Security and Bio-Economy
- Ectopic Expression of PtCYP714A3 from Populus trichocarpa Improves Salt Tolerance in Transgenic Rice
- Kn1 Gene Overexpression Improves Transformation Efficiencies of Citrus Cultivars
- OsBBX14 Delays Heading Date in Rice
Beyond Crop Biotech
- Abiotic Stress Tolerance Genes Characterized in Brown Seaweed
- Scientists Develop GM Microbe for Monogastric Animal Nutrition
- Researchers Discover MicroRNA that may Protect from Obesity and Diabetes
- Genome Editing and Gene Modulation Congress 2016
- 3rd Plant Genomics Congress: Asia
- 5th International Conference and Exhibition on Metabolomics
Read the latest:
- Biotech Updates (March 29, 2023)
- Genome Editing Supplement (March 22, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: