Expression of Silencing-Suppressor Protein Enhances the Performance of an Engineered Metabolic PathwayDecember 16, 2015
Expression of a transgene can dwindle over time due to the plant's silencing pathways. These have evolved as an antiviral defense, and viruses have co-evolved viral silencing-suppressor proteins (VSPs) to block them. VSPs have then been used with transgene constructs to enhance its expression. However, it usually causes developmental abnormalities.
Fatima Naim of the University of Sydney in Australia, together with her colleagues evaluated a number of different VSPs expressed in the seeds of Arabidopsis thaliana alongside a transgenic pathway for arachidonic acid (AA), polyunsaturated fatty acid.
Results from independent transgenic events, maintained for four generations, showed that the VSP-AA-transformed plants were normal and could produce 40% more AA than plants transformed with the AA transgene alone. A geminivirus VSP, V2, was also found to be expressed without causing developmental defects and gave strong transgene enhancement. These results demonstrate that VSP expression can be used to protect and enhance stable transgene performance.
For more information, read the article in Plant Biotechnology Journal.
The Crop Biotech Update is a weekly newsletter of ISAAA, a not-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Paris Climate Agreement Recognizes Food Security as a Priority
- Nobel Laureate: GM Crops Vital to Address Malnutrion
- Farmers in Western Kenya Request for Speedy Commercialization of GM Cotton
- Kenya National Biosafety Authority Receives Public Comments on Bt Cotton Environmental Release Application
- Zambia National Biosafety Authority Launched
- Scientists Sequence Walnut Genome
- Vietnam Sets New Rules on Labeling GM Foods
- China Shares Biotech Commercialization Experience with Developed Countries
- First Map of Wheat Epigenome Created
- Characterization of the Stress-inducible PLATZ Gene from Soybean
- JMJ704 Protein Regulates Rice Defense Response Against Xanthomonas oryzae pv. oryzae
- Expression of Silencing-Suppressor Protein Enhances the Performance of an Engineered Metabolic Pathway
Beyond Crop Biotech
- Enzyme-Producing GE Chicken Gets Approval in the US
- Genus and University of Missouri Develop Pigs Resistant to Incurable Disease
From the BICs
- IndoBIC Conducts Visit to Corn Research Stations in Malang, Indonesia
Read the latest:
- Crop Biotech Update (November 23, 2022)
- Genome Editing Supplement (November 23, 2022)
- Gene Drive Supplement (October 26, 2022)
Subscribe to CBU: