Crop Biotech Update

Tobacco NsylCBL10 Overexpression Enhances Salt Tolerance in Arabidopsis

November 11, 2015

The calcineurin B-like (CBL) proteins are plant calcium sensors which play an important role in regulating the stress response of plant cells. Researchers, led by Lianhong Dong of the Chinese Academy of Agricultural Sciences, studied its function in CBL tobacco (Nicotiana sylvestris).

NsylCBL10, a CBL gene, was cloned from tobacco. NsylCBL10 was found to be expressed in most tissues. Overexpression of NsylCBL10 in Arabidopsis enhanced the salt tolerance of plants greatly, and rescued the salt-sensitive mutant, Arabidopsis cbl10. Analysis reveals that plants overexpressing NsylCBL10 were able to maintain a lower Na+/K+ ratio in roots and higher Na+/K+ ratio in shoots.

Results suggest that NsylCBL10 may play an important role in tobacco's response to high salinity stress, by keeping a better ionic homeostasis to reduce damage to plant cells.

For more information, read the article in Plant Cell Reports.