Species: Chicken (Gallus gallus)

Country of 1st Author: South Korea
Year: 2020

Institute	Seoul National University, Pyeongchang-gun Gangwon-do KR
Editing Method	Other
SDN Type	SDN-1
Delivery	Lipofectamine 3000 reagent was diluted in 250 µL of OPTIMEM (Invitrogen), and 2.5 µg each D10A-Cas9-GFP coexpression plasmid and two MSTN guide RNAs (gRNAs) were mixed with Lipofectamine P3000 reagent in 250 µL of OPTI-MEM at room temperature.
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
MSTN Myostatin	Double muscle	Yield	Other	we utilized D10ACas9 nickase to generate myostatin-knockout (MSTN KO) chickens via primordial germ cells.

References, Documents, and Links

• Generation of myostatin-knockout chickens mediated by D10A-Cas9 nickase