Species: Cattle (Bos taurus)

Country of 1st Author: China
Year: 2015

Breed Strain	Holstein-Friesian
Institute	Northwest A&F University Yangling 712100, Shaanxi CN
Editing Method	Cloned cell line
SDN Type	SDN-3
Delivery	Nine of the transgenic cell colonies were used as donor cells to produce cloned transgenic cattle.
Foreign DNA	Yes
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
SP110 Mouse SP110	Resistance to tuberculosis	Biotic Stress	TALEN	TALENs specific to the intergenic region between surfactant protein A1 (SFTPA1) and methionine adenosyltransferase I alpha (MAT1A) on chromosome 28. EGFP or mCherry was added to the TALEN vectors with a self-cleaving T2A peptide to sort transfected cells

References, Documents, and Links

• TALE nickase-mediated SP110 knockin endows cattle with increased resistance to tuberculosis