Species: Pig (Sus scrofa domesticus)

Country of 1st Author: China
Year: 2016

Breed Strain	Large White
Institute	Jilin University, Changchun Jilin Province CN
Editing Method	Cloned cell line
SDN Type	SDN-2
Delivery	Donor cells heterozygous for intended mutation were cloned
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
MSTN Myostatin	Double muscle	Yield	CRISPR Cas9 (spCas9)	Knockin Orthologous Point Mutation to disrupt protein function via CRISPR-assisted ssODN-mediated Homology-directed repair

References, Documents, and Links

• Efficient Generation of Orthologous Point Mutations in Pigs via CRISPR-assisted ssODN-mediated Homology-directed Repair