Utilization of PVX-Cre Expression Vector in Potato
October 7, 2011| |
Trait genes are introduced into the plant genome together with a marker gene. After transgene selection and characterization, the marker gene becomes unimportant. To develop transgenic plants free from selectable marker, site-specific recombination is performed. Lilya Kopertekh of the Federal Research Centre for Cultivated Plants (JKI), Germany, together with other scientists used the transient Cre-lox system to remove the nptII marker gene from potato.
Cre-lox system involves introduction of Cre protein lox-target plants by PVX virus vector followed by plant regeneration. Through particle bombardment and a silencing suppressor protein, 20-27 percent of the regenerated plants were found to be marker-free. After comparing the recombination frequencies in this study and the other methods usually used to remove marker gene in potato, it is suggested that PVX-Cre mediated site-specific excisional recombination is a useful tool to generate potato plants without superfluous transgenic sequences.
Read the abstract at http://www.springerlink.com/content/25x42557083g61h0/.
| |
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- Policy Implications to Advance Development and Adoption of GM Technology
- Cereal World Production Up But Price Decline Anticipated
- Agroecology Offers Model for "New Green Revolution"
- Ghanaians Urged to Embrace Biotech for Food Security
- Infrastructure for Wheat Rust Trials in Kenya
- Spotlight on Biofortified Crops during the FANUS Conference
- African Rice Experts Adopt a New Way of Doing Business
- How Experts and Non-Experts Make Decisions on GM Crop Release
- Plant Biologists Seek Solutions to Global Food Concerns
- Developing Drought Tolerant Corn Hybrids
- UC Davis Scientist Discusses Biotech to Journalism Students at MU
- Asian Regional Workshop on Sustainable Agriculture, Biotechnology and Biosafety
- Kendall Urges Industry to Promote GM Wheat Benefits
- India to Establish Borlaug Institute for South Asia
- ACIAR Study: Rice Breeding Boosts Yields and Farmers' Income
- Workshop on Potato Breeding Concluded in Makassar, Indonesia
- JIC Scientists Open Eyes on Stomatal Spacing
- Biotechnology, Necessary to Climate Proof Crops
- DFID to Support Delivery of Nutrient-rich Crops
- Lobby Groups Threatening Public Acceptance of GM Technology
- Defeating Nematode Worms with GM Bananas
- New Insight Into Plant Immune Defenses
-
Research Highlights
- Impacts of Bt Cotton on a Non-target Pest, Apolygus lucorum, in Northern China
- Scientists Compare Proteomic Profiles of MON810 Maize Varieties and their Non-GM Varieties
- Utilization of PVX-Cre Expression Vector in Potato
-
Beyond Crop Biotech
- Genome Reveals Blood Pressure Markers
-
Announcements
- 2012 Weed Science Society of America: Annual Meeting
- Second Conference on System Biology and New Sequence Techniques
- Functional Genomics and System Biology, Cambridge, UK
-
Read the latest: - Biotech Updates (April 24, 2024)
- Gene Editing Supplement (April 24, 2024)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet