Researchers Fine-Tune CRISPR-Cas9 Gene Editing in Common Bean
October 25, 2023| |
A study from Frontiers in Plant Science shows that the hairy root transformation system allowed a rapid evaluation of multiple single guide RNA (sgRNA) and promoters in common beans (Phaseolus Vulgaris L.). Results also indicate that the Lindel repair model is deemed the most effective tool for predicting sgRNA efficiency and the type of induced mutation in most hairy roots in common beans.
Common bean is a good source of dietary protein and other essential nutrients. However, recent advancements in plant biotechnology using CRISPR-Cas9 are prone to error and would edit a few base pairs in small numbers. Furthermore, the currently available bioinformatic programs for designing sgRNAs, CRISPOR and RGEN, have limited applications to plant systems. Hence, the researchers developed a rapid and efficient screening method to validate sgRNA efficiency in common beans.
The results of this research accelerate the goal of developing stable transgenic lines of common beans with improved nutritional content for human and animal consumption.
For more information, read the article from Frontiers in Plant Science.
| |
You might also like:
- Transgenic Common Bean Offers More Sustainable Strategy Against Whitefly
- Transgenic Common Bean Lines Show Multiple Resistance to Three Viruses
- Effect of Drought Stress on Photosynthate Allocation and Remobilization in Common Bean Pods
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
Gene Editing Supplement (October 25, 2023)
-
Research and Tools
- New Insights into CRISPR-Cas9 sgRNA Design for Plants
- Gene Editing in Rice Using CRISPR-Cas12i3
- CRISPR Helps Researchers Understand Mutations in Tomato
- Researchers Fine-Tune CRISPR-Cas9 Gene Editing in Common Bean
- Review of CRISPR to Boost Resistance to Parasitic Plants
- Researchers Develop Software Tool for Safer Gene Editing Design
-
Read the latest: - Biotech Updates (April 24, 2024)
- Gene Editing Supplement (April 24, 2024)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet