Crop Biotech Update

CRISPR Used to Increase Glucoraphanin Content in Chinese Kale

May 24, 2023

Researchers from Sichuan Agricultural University, China, used gene editing to improve the nutritional value of Chinese kale. The results are published in Food Research International.

Plant secondary metabolite glucoraphanin (GRA) generates a hydrolysis product that has potent anticancer activity. GRA is available in Chinese kale but only in trace amounts. To improve the GRA content of Chinese kale, three copies of the ALKENYL HYDROXALKYL PRODUCING 2 (AOP2) gene were isolated and edited using the CRISPR-Cas9 system. This led to boaaop2 mutants with increased levels of GRA. It was also observed that the expression of upstream genes of glucosinolate biosynthesis was decreased in the mutants.

Based on the findings, the researchers pinpoint BoaAOP2.1 as an effective gene for the alkenylation of aliphatic glucosinolates.

Read the research article in Food Research International.

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