Expression of Cadherin, APN, and ALP Genes in Diamondback Moth StrainsNovember 11, 2011
Cadherin proteins, aminopeptidase N (APN), and alkaline phosphatase (ALP) bind to Bacillus thuringiensis (Bt) toxins in the lepidopteran midgut. However, the intrinsic functions of these receptors are not yet known. Z. -X. Yang of Hunan Agricultural University in China, together with other scientists, conducted a study to investigate if these receptors contribute to the resistance of lepidopteran insects to the Bt toxin.
Using real-time quantitative polymerase chain reaction, they examined the transcription patterns of the genes expressing the receptors in Bt toxin-susceptible diamondback moth (Plutella xylostella) strains and strains with high resistance to Bt toxin. The gene expressions of cadherin and APNs were found to be high in the midgut of fourth larval instars and present in the pupae. The transcription levels of cadherin, APN2, and ALP genes in the midgut were more abundant in the susceptible strain compared to the resistant strain. The transcription levels of cadherin increased as the insect developed.
These findings suggest that the differences in expression may be attributed to the adaptation of the resistance strain to the Bt toxin. The results also imply a possible relationship between the gene expression of cadherin, ALP, and APN2 and the resistance of diamondback moth to Bt.
Read more information at http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0418.2011.01683.x/abstract.
The Crop Biotech Update is a weekly newsletter of ISAAA, a not-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Getting a Biotech Crop to Market
- G20's Commitment to Restore Global Growth
- FAO Food Price Index Down in October
- Agricultural Conference Aims to Increase Food Production in Africa
- African Initiative to Take Leadership of Research on Key Crops
- Argentina Approves New GM Corn
- Pioneer Hi-Bred Introduces 33 New Soybean Products for 2012
- Weed Identification Guide Available Electronically
- Diversity of Cabbage Species Explained
- JGI Selects 41 Projects for 2012 Community Sequencing Program
- Coffman Receives 2011 Mentoring Award
- Genome Sequence of Pigeonpea, an Orphan Legume Crop decoded
- Seminar on Liability and Redress for Damage to Biological Diversity in China
- French Seed Industry Association's Call for Freedom to Innovate
- EFSA on Placing on the Market GM Maize MON 88017
- Knowledge from Science to Societies: The KNOSSOS Project
- JIC Scientists Study How Parasites Modify Plants to Attract Insects
- Genetic Dissection of Biotic Stress Response Using RiceNet
- Gene Expression Profile Changes in Germinating Rice
- Expression of Cadherin, APN, and ALP Genes in Diamondback Moth Strains
Beyond Crop Biotech
- FSANZ Invites Public Comment on Use of GM-derived Enzyme
- Eat Strawberries to Combat Alcohol's Effects on Stomachs
- Functional Genomics and System Biology, Cambridge, UK
- Specialty Crop Regulatory Assistance Workshop
- Report of the International Symposium on COP 10 and COP-MOP 5 Outcomes, Tokyo
Read the latest:
- Crop Biotech Update (October 5, 2022)
- Genome Editing Supplement (October 5, 2022)
- Gene Drive Supplement (September 28, 2022)
Subscribe to CBU: