Development of a Construct-specific PCR Detection Method of GM Phytase Maize Line
June 10, 2011| |
Transgenic phytase maize line BVLA430101 was the first GM maize that obtained the security certification in 2009 in China, but the construct of the gene expression and the specific detection method have not been reported yet. Thus, Changqing Su of the Chinese Academy of Agricultural Sciences and colleagues identified and described the phytase gene expression cassette. The construct-specific qualitative and quantitative PCR methods of BVLA430101 maize were derived based on the transition of signal peptide and phytase gene using a maize taxon-specific gene zSSIIb as the endogenous gene. Moreover, two known BVLA430101 contents of mixed genomic DNA were quantified using the developed real-time PCR detection system, which showed that the developed quantitative method can be used for GM phytase maize BVLA430101 measurement.
Subscribers of the European Food Research and Technology Journal may view the research article at http://www.springerlink.com/content/d0m82167k4221676/.
| |
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- Hotter Summers Beginning in 20 Years, says Stanford Climate Scientists
- World Food Prices Set to Remain High
- Southern African Confederation of Agricultural Unions Supports GM Crops in Africa
- Research Partnership to Reduce Crop Loss From Striga Weed in Africa
- G8-Africa Partnership to Revitalize Agriculture
- Uganda Implements Field Trials of GM Bananas
- Farmer Networks Crucial in Adoption of New Technologies
- Scientists Shed Light on Phototrophism in Dicots
- First Company Accreditation by USDA Seed Conditioning Program
- Indonesian Government Officials and Farmers Briefed on Biotech
- ITIF Fellow Updates Indonesians on Biotech Realities in the EU
- South-South Cooperation and Biotech in Asia
- Kenyan National Biosafety Authority CEO Shares Status of the Country's Regulatory System
- Bt Eggplant Vandals in the Philippines to be Prosecuted with Malicious Mischief
- Urgent Call to G20 Global Leaders to Continue Investments in Agricultural Development
- Project to Improve Drought Tolerance of Potatoes
- Photosynthesis and Climate Change
- Experts Search for Sources of E. coli in Farms
-
Research Highlights
- Performance and Milk Composition of Cows Fed with Bollgard II Cottonseeds
- Scientists Investigate the Levels of Control of Stem Borer in Bt maize in Kenya
- Development of a Construct-specific PCR Detection Method of GM Phytase Maize Line
-
Beyond Crop Biotech
- Deadly Bacteria May Mimic Human Proteins to Evolve Antibiotic Resistance
- Nanoparticles in Food Crops Need to be Studied
- Biomaterial from Tamarind Seeds Aids Nerve Regeneration
-
Resources
- The Trends and Future of Biotechnology Crops for Insect Pest Control
- New Publication on Pesticides, Latest WHO Ranking, Regulation and Label Claims in India
- GM Crop Production in Canada: Agronomic, Ecological, and Environmental Considerations
-
Read the latest: - Biotech Updates (April 23, 2025)
- Gene Editing Supplement (April 16, 2025)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet