Biotech Updates

Protein Engineering of Trichoderma reesei Anamorph for Improved Cellulases

September 24, 2010
http://www.biotechnologyforbiofuels.com/content/3/1/20
http://www.biotechnologyforbiofuels.com/content/pdf/1754-6834-3-20.pdf

Scientists from Genencor (a major biotechnology company) and the Swedish University of Agricultural Sciences, report some research on the protein engineering of Hypocrea jecorina (an anomorph, or asexual reproductive stage, of Trichoderma reesei), a major cellulose-producing fungus. The objective is to eventually obtain a formulation of cost-effective cellulases for the saccharification step in the ethanol production process from lignocellulosic biomass. In the first phase, they mentioned that they subjected some components of the cellulase-enzyme-system (called CEL7A and CEL6A cellobiohydrolases) to protein engineering, to improve the enzyme's thermostability. In the next phase, they moved towards "improving CEL6A- and CEL7A- specific performance in the context of a complete enzyme system under industrially relevant conditions". In the present study, they report their advances mainly on the research activities involving CEL6A, in the open access journal, Biotechnology for Biofuels (URL above). For related information on teleomorph, anamorph and holomorph and the naming of fungi, see http://en.wikipedia.org/wiki/Anamorph. See also Trichoderma Systematics, Sexual State and Ecology (USDA publication in Phytopathology journal) at http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-96-0195