Biotech Updates

MitoTALENs Restore Fertility of Ogura Cytoplasmic Male Sterile Broccoli

January 17, 2024

A study published in the Plant Biotechnology Journal shows that the knockout of ORF138 has restored the fertility of cytoplasmic male sterility (CMS) derived broccoli hybrids. The results of this study provide significant insights into the role of mitochondria in sex production machinery and the temperature-responsive ability of vegetable crops.

The production of hybrid crops has been dominating the global seed markets because of the observed significant improvement in crop yields. CMS, a trait that prevents the production of viable pollen grains due to mitochondrial dysfunction, has been used to produce hybrid crops. In this study, the researchers used mitochondrial targeted transcription activator-like effector nucleases (mitoTALENs) to knockout ORF138 in a CMS-derived broccoli hybrid.

The findings of the study indicate that the restoration of fertility was observed in high temperatures. The depletion of ORF138 was also observed to be stably inherited by the next generation. With the ORF138 knockout line, the researchers were also able to identify several hub genes that may contribute to mitochondrial function, fertility restoration, and flower development.

For more information, read the article from Plant Biotechnology Journal.

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