Advantages and Challenges of Using CRISPR-Cas to Target miRNA in Cancer
July 19, 2023| |
Researchers from various universities in Iraq analyzed the different benefits and challenges of using CRISPR-Cas to target miRNA in Cancer. They also offered advanced strategies that can be used to overcome potential issues.
CRISPR-Cas can be used to insert permanent mutations in the genome that can be transferred to the next generation of cells. Other benefits of this technique include higher accuracy and specificity compared to other methods, as well as the targeting of several loci in the miRNA gene. CRISPR-Cas approach for miRNA gene knockdown also reaches a high success rate of 75%-99% with minimal off-target activity.
The challenges of CRISPR-Cas miRNA targeting that need to be addressed include Cas9 PAM deficiency in miRNA sequence, the short sequence of miRNA, off-target outcomes in CRISPR-Cas-mediated miRNA editing, and delivery issues of the CRISPR-Cas system. The researchers provided different strategies to help resolve these issues.
Read the journal article at Military Medical Research for more information.
| |
You might also like:
- Study Shows Link between CRISPR and Mutated Cancer Cells
- Scientists Report First use of CRISPR to Substitute Genes to Treat Patients with Cancer
- Study Finds CRISPR-Cas9 Leads to Unexpected Genomic Changes
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
Plant
- Philippine Judiciary Members Increase Knowledge and Capacity Through Biotech Webinar
- Natural Tomato Mutation Found to be Related to BER Resistance
- Researchers Breed Poplar with Less Lignin Using CRISPR Gene Editing
- GE High Antioxidant Purple Tomato Completes FDA Consultation
- Scientists Visualize Activity of CRISPR Genetic Scissors
- ISAAA Launches Resource Page on TALENs
- ISAAA Inc. to Hold ASCA6 on September 11-15 in Indonesia
-
Food
- Hunger Levels Reach Record High, 735 Million People Struggling
-
Health
- Advantages and Challenges of Using CRISPR-Cas to Target miRNA in Cancer
-
Read the latest: - Biotech Updates (October 9, 2024)
- Gene Editing Supplement (September 26, 2024)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet