CRISPR-Cas9 Technology Reveals Function of OsRhoGDI2May 6, 2020
Henan Normal University scientists used CRISPR-Cas9 to identify the function of OsRhoGDI2 in rice plants. The results are published in the Chinese Journal of Biotechnology.
OsRhoGDI2 was isolated as a putative partner of Rho protein family member from rice panicles by yeast two-hybrid, but its role is still unclear. Thus, Kaijie Wang and the team developed OsRhoGDI2 knockout mutants using CRISPR-Cas9 technology. Sequence analysis of the mutants showed base substitution or base deletion which occurred close to the editing targets of the gene in knockout rice. Analysis of the physical characteristics showed that OsRhpGD12 knockout rice plants had lower plant height than the control plants. This was further confirmed by statistical analysis, which indicated that the significant reduction in plant height was evident in the second internodes. These findings suggest that the OsRhoGDI2 gene is linked with the regulation of rice plant height.
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