DNA-free Genome Editing Using Preassembled CRISPR-Cas9 with Ribonucleoproteins
July 31, 2019| |
Researchers from Naturegenic reported a new method for precise genome engineering using a preassembled CRISPR-Cas9 ribonucleoprotein (RNP) complex. Traditional trait development used to depend on genetic variations from spontaneous or artificial mutation, however, these methods lead to low probability of generating the desired traits. The new method reported in Transgenic Research journal showed to be more precise and can be used among different species without leaving any foreign DNA footprint in the genomes.
The researchers initially obtained protoplasts after removing the plant cell wall, then the RNP was introduced into the plant nucleus. Whole plants were regenerated from single cell of protoplasts engineered with RNP. Results confirmed successfully engineered plant genomes without traces of foreign DNA.
The results of the study could help develop new traits in crop plants using protoplast regeneration technology safely and precisely in less time.
Read more details in Transgenic Research.
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