Blight Disease Resistance of TBR225 Rice VarietyApril 10, 2019
OsSWEET family encodes sugar transporters which are involved in the bacterial leaf blight (BLB) disease. In a study published in the Journal of Agriculture and Rural Development, promoter OsSWEET14 was isolated from Xanthomonas oryzae pv. oryzae (Xoo) susceptible TBR225 rice variety. The 1392-bp isolated DNA sequence contains four cis acting elements recognized specifically by Xoo TAL proteins, including TalC, Tal5, PthXa3 and AvrXa7. OsSWEET14-TBR225 showed the similarity of 99% and 100% to OsSWEET14 of GeneBank-registered Japonica (Niponbare, AP014967.1) and Indica (Shuhui498, CP018167.1) varieties, respectively. Based on the isolated DNA sequence, three gRNAs were designed for modifying the TAL effector binding sites on the OsSWEET14 promoter and knockouting the OsSWEET14 gene using CRISPR-Cas9 technology in order to enhance the resistance of TBR225 rice veriety.
The research can be used as basis for generating BLB disease resistant and high yielding rice varieties using genome editing technology in Vietnam.
For more information, read the article in Vietnamese in the Journal of Agriculture and Rural Development.
The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Durum Wheat Genome Decoded
- Scientists Use Plant Hormones to Fight a Parasitic Crop Pest in Africa
- The Search for New Sources of Salt Tolerance in Carrots
- Scientists Pinpoint Gene Vital for Making Maize Ears
- Maize Yields Influenced by Unexpected Gene 'Moonlighting'
- Scientists Develop Model that Predicts Photosynthetic Manipulations to Increase Yields
- Agri-biotech Stakeholders Convene for Pan-Asia Farmer's Exchange Program
- Two Studies Reveal Steps in Plant Immune Receptor Activation
- EFSA Releases Scientific Opinion on Six-Event GM Maize
- Study Explains Why Plants Grow Less in Hot Environments
- Vietnamese Scientists Develop Drought Tolerant Rice Lines
Plant Breeding Innovations
- Blight Disease Resistance of TBR225 Rice Variety
- Study Shows Single and Multiple Gene Knockouts by CRISPR-Cas9 in Maize
Beyond Crop Biotech
- Scientists Genetically Engineer Yeast to Improve Understanding of How Cells Work
- Register to Plant Genomics and Gene Editing Congress: Asia (10% Discount for CBU Subscribers)
Subscribe to CBU: