CRISPR-Cas9 Used in Breeding Indica Glutinous CMS Line WX209A
December 19, 2018 |
Glutinous cytoplasmic male sterile (CMS) line is vital for the selection of hybrid glutinous rice combination with high yield and quality. To develop glutinous CMS with low amylose content, researcher Xin Wang and team knocked out the granule-bound starch synthase OsWaxy in 209B using CRISPR-Cas9-mediated genome editing technology and successfully obtained a glutinous maintainer line WX209B.
When compared with maintainer line 209B, WX209B exhibited reduced amylose contents and similar agronomic characteristics. After one generation of hybridization and two generations of backcrossing with WX209B as the male parent and 209A as the female parent, the glutinous CMS line WX209A was successfully achieved.
The results of the study provide a strategy to efficiently breed for the glutinous cytoplasmic male sterile line by combining CRISPR-Cas9-mediated gene editing technology with conventional backcross breeding method in a short period, which prepares the ground for further breeding of hybrid glutinous rice variety.Read the research article from Czech Academy of Agricultural Sciences.
|
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- Multidisciplinary Study Traces Movement of Maize in South America
- Rwandan Women in Biosciences Embrace Science Communication
- Iowa State University Study Says Anti-GMO Sentiment Has Repercussions for Developing World
- Scientists Discover Way to Make Rice Plants Replicate through Seeds as Clones
- Australian OGTR Receives License Application for Field Trial of GM Chickpea
- Japan May Allow Genome-edited Food Sale
- Plants Don't Like to be Touched, Study Finds
- Stockholm University Scientists Discover Gene that Helps Submerged Plants
-
Research Highlights
- Research Reveals MON 810 and NK603 GM Maize Have No Effects on Rat Health or Metabolism
- Study Shows Inheritance of Transgenes in Bt Cotton Lines Resistant to Bollworm
-
Beyond Crop Biotech
- GM Pigs Resist Infection from Classical Swine Fever Virus
-
Resources
- Revised ISAAA Infographics: 22 Years of Biotech Crops in the World
- Talking Biotech: How Do We Get Consumers to Think Like Scientists?
-
Plant
- Europe Grants CRISPR-Cas9 Patent to Calyxt
- CRISPR-Cas9 Used in Breeding Indica Glutinous CMS Line WX209A
-
Read the latest: - Biotech Updates (October 30, 2024)
- Gene Editing Supplement (October 30, 2024)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet