New Research Could Fine-tune CRISPRDecember 5, 2018
As researchers and scientists use the tool CRISPR to correct genetic errors, they are aware that it may have side effects on the human genome. Now, researchers from the University of Copenhagen have learned how the molecular machinery behind CRISPR works and thus, expect to be able to fine-tune CRISPR and remove the undesired effects.
In a new study published in Cell, researchers from the Novo Nordisk Foundation Center for Protein Research described how one of the CRISPR technologies, Cas12a, works all the way down to the molecular level, making it possible to fine-tune the gene-editing process. The researchers mapped the technology and took photographs of the different shapes of the molecule when CRISPR-Cas12a cuts up the DNA strand. They combined it with a technique called ‘single molecule FRET' that directly observes the motions of the molecules and the sequence of events for each individual protein. This sequence of events revealed that three "pieces" of the CRISPR tool must change form for the DNA to be cut properly.
The researchers said that their findings can explain why CRISPR can have side effects on the genome. Once the DNA strand has been cut, the three ‘security checks' remain open, which make the process last longer than wanted, because the machinery behind gene editing continues to run and can cause genetic changes. The researchers expect their new knowledge to put an end to this, and fine-tune the gene-editing technology right away.
For more details, read the news article from the University of Copenhagen.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- FAO DG Calls for Countries to Address All Forms of Malnutrition
- Governments Set Targets for Biodiversity Conservation by 2050
- Uganda Parliament Passes GMO Bill
- Research Finds Autophagy's Remarkable Influence on Plant Metabolism
- Guatemala and Honduras Send Draft Biotechnology Regulation to WTO
- Colombia Open to Biotech Adoption; Continues to Work through Regulatory Challenges
- International Team Discovers Why Plants "Live Fast and Die Young"
- Inactivating Genes Boosts Crop Genetic Diversity
- PAC1 Overexpression Improves Multiple Virus Resistance of Soybean
- Scientists Reveal How Plants Sense Temperature
Beyond Crop Biotech
- Study Identifies Geographic Origins of Hazelnuts
- New Research Could Fine-tune CRISPR
- 20 Years of GMOs in Brazil
- Updated ISAAA Infographics: Where are Biotech Crops Grown in the World?
- Researchers Use CRISPR-Cas9 for Gene Editing of Cavendish Banana
- Gene-edited Rice Shows BIG Gene Vital for Seedling Viability
Read the latest:
- Biotech Updates (September 27, 2023)
- Gene Editing Supplement (September 27, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: