RNAi Improves CRISPR-Cas9 Efficiency and Selection
October 3, 2018 |
Higher expression of CRISPR-Cas9 components allows higher gene editing frequency, reducing the cost of producing mutants for screening. Moreover, selection through the naked eye also reduces the cost of selecting mutated plants through other molecular techniques. Overall, the system provides practicality in increasing gene expression and selecting transgene-free CRISPR-Cas9-edited plants in the first and second generations by naked-eye observation of symptom severity.
For more information, read the article in BMC Genome Biology.
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