Researchers Use Ribonucleoprotein Complexes for Genome Editing of WheatMarch 7, 2018
CRISPR-Cas9 DNA constructs are transformed into cells via Agrobacterium-mediated transformation. However, this process is associated with a high possibility of CRISPR-Cas9 DNA constructs being integrated into the plant genome, causing unwanted genetic changes. Aiming to avoid this, Zhen Liang of Chinese Academy of Sciences developed a genome editing method for bread wheat (Triticum aestivum) using CRISPR-Cas9 ribonucleoproteins (RNPs).
The team successfully generated five mutants from immature wheat embryos. Analysis reveals that the chance of off-target mutations in wheat cells was much lower in RNP-mediated genome editing than in editing using DNA. No off-target mutations were also detected in the mutant plants.
Since no foreign DNA was used in RNP-mediated genome editing, the mutants obtained were completely transgene free.
For more information, read the article in Nature Communications.
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