Researchers Use Ribonucleoprotein Complexes for Genome Editing of Wheat
March 7, 2018 |
CRISPR-Cas9 DNA constructs are transformed into cells via Agrobacterium-mediated transformation. However, this process is associated with a high possibility of CRISPR-Cas9 DNA constructs being integrated into the plant genome, causing unwanted genetic changes. Aiming to avoid this, Zhen Liang of Chinese Academy of Sciences developed a genome editing method for bread wheat (Triticum aestivum) using CRISPR-Cas9 ribonucleoproteins (RNPs).
The team successfully generated five mutants from immature wheat embryos. Analysis reveals that the chance of off-target mutations in wheat cells was much lower in RNP-mediated genome editing than in editing using DNA. No off-target mutations were also detected in the mutant plants.
Since no foreign DNA was used in RNP-mediated genome editing, the mutants obtained were completely transgene free.
For more information, read the article in Nature Communications.
|
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- Women Play Vital Role in Biotech, Study
- Ugandan Farmer Requests for Access to Beneficial Farming Technologies
- First Zinc-enriched Maize Released in Colombia to Combat Malnutrition
- Biofuels from Plant Fibers Could Fight Global Warming
- UGA Researchers Design New Technique to Enhance Crops
- Brazil Sugar Mills Start Planting GM Sugarcane
- Argentina Approves Three GM Crops
- EFSA Publishes Risk Assessment for Renewal of GM Maize Authorization
- Researchers Use NBTs to Develop Tobacco Plants as Biofactories
-
Research Highlights
- Host-Induced Gene Silencing of VdRGS1 Confers Resistance to Verticillium Wilt in Cotton
- Rice Genes OsBON1 and OsBON3 Suppress Broad-Spectrum Disease Resistance
-
Beyond Crop Biotech
- Kenya Developing Animal Biotech Regulatory Guidelines
-
Announcements
- Future of Long-term Experiments in Agricultural Science
-
Resources
- 5 Questions with UCT's Jennifer Thomson
-
Plant
- Knock-out of Zmsweet13 Genes Impairs Agronomic Traits in Maize
- CRISPR-Cas9 Helps Explain Maize Adaptation to Higher Latitudes
- Blocking OsAAP3 in Rice Improves Grain Yield
- Researchers Use Ribonucleoprotein Complexes for Genome Editing of Wheat
-
Read the latest: - Biotech Updates (October 30, 2024)
- Gene Editing Supplement (October 30, 2024)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet