Optimized CRISPR-Cas9 Induces Targeted Mutation in Kiwifruit
January 24, 2018| |
Kiwifruit (Actinidia deliciosa) is an important fruit crop but has limited technologies for its genetic improvement. The CRISPR-Cas system has been successfully used in many crops, but it has not been used in kiwifruit. Furthermore, optimizing the conditions for its use in a particular plant species is needed to achieve efficient genome editing.
The team of Zupeng Wang from the Chinese Academy of Sciences aimed to apply CRISPR-Cas in kiwifruit. The team created a synthetic guide RNA (sgRNA)-Cas9 vector that targets the kiwifruit phytoene desaturase gene (AcPDS). Albino kiwifruit plants were successfully obtained, a phenotype caused by a knocked-out AcPDS gene.
The vectors used were compared with other vectors that contain different sgRNA. While all vectors can induce albino phenotype of kiwifruit, they found that the polycistronic tRNA-sgRNA cassette (PTG-Cas9) system was significantly more efficient in kiwifruit genome editing.
This study proves that the CRISPR-Cas9 system can be applied in kiwifruit genome editing. The sgRNA in this system can also be optimized, as exemplified by the PTG-Cas9, and can provide clues for optimizing CRISPR-Cas9 system in other plants.
For more information, read the article in Plant Biotechnology Journal.
| |
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- Nigerian Farmers May Soon Plant Bt Cowpeas
- Scientists Recommend Bt Maize as Solution to Fall Armyworm Infestation in Kenya
- U.S. FDA Approves China's GE Rice
- Study Finds that Hormone Keys Plant Growth or Stress Tolerance, But Not Both
- Plant Biologists Develop Synthetic Auxin to Understand Plant's Mysteries
- Scientists Find "Thermostat" in Plant Immunity
- Wild Rice from Crocodile-Infested Waters in Australia May Help Boost Global Food Security
- Study Reveals More Genes are Active in High-Performance Maize
- EU Court of Justice Advocate General Says Gene Edited Crops Should be Exempted from GM Food Laws
-
Research Highlights
- Apple Protein MdY3IP1 Triggers Early-Flowering and Salt-Tolerance in Arabidopsis
- Scientists Reveal the Role of SlJAZ2 in Tomato
-
Beyond Crop Biotech
- CmHSFA4 Positively Regulates Salt Stress Tolerance in Transgenic Chrysanthemum
-
Announcements
- 5th International Rice Congress
-
Plant
- Optimized CRISPR-Cas9 Induces Targeted Mutation in Kiwifruit
- Researchers Apply CRISPR-Cas9 on Carrot Cells
-
Read the latest: - Biotech Updates (April 17, 2024)
- Gene Editing Supplement (April 10, 2024)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet