CRISPR/Cas9 Genome Editing of Rubber-Producing DandelionNovember 16, 2016
Rubber dandelion (Taraxacum kok-saghyz; TK) is notable for its ability to produce rubber in its roots and a potential alternative source of natural rubber. In order to accelerate the domestication of TK, Brian Iaffaldano and his team from Ohio State University have established a simple strategy to use CRISPR/Cas9 in this species to target the fructan:fructan 1-fructosyltransferase (1-FFT) gene, implicated in inulin synthesis. This gene was targeted as inulin is an expected antagonist of rubber production.
TK plantlets were inoculated with Agrobacterium rhizogenes harboring a plasmid encoding a Cas9 and single guide RNA targeting TK 1-FFT. The team was able to rapidly induce hairy roots harboring knockout alleles. Mutagenesis was confirmed by observing a loss of restriction sites within 1-FFT.
Out of 11 hairy root samples, 10 showed the presence of genome editing, with mutation rates as high as 88.9%, suggesting a high efficiency mutagenesis induced by CRISPR/Cas9 via A. rhizogenes-mediated transformation. Whole TK plants were then generated from hairy roots harboring knockout alleles. The regenerated plants contained knockout alleles, with mutation rates as high as 80.0%.
The application of high efficiency CRISPR/Cas9 genome editing could facilitate the rapid domestication and commercialization of TK as a rubber producing crop, and may accelerate basic research on the regulation of rubber synthesis.
For more information, read the article in Industrial Crops and Products.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Global Ban on GM Crops to Raise Food Prices, Add A Billion Tons of CO2 to Atmosphere
- Half of South Africans Believe GM Crops are Good for the Economy
- Scientists Identify Gene to Fight Wheat Scab
- UF/IFAS Study Says People Who Know About GM Foods Agree with Science that They are Safe
- Indian Farmers Benefit from GM Crops
- Survey Shows Agriculture Extension Officers in Pakistan Need More Training on Biotech
- Scientists Develop Marker-free GM Hexaploid Wheat
- Wheat Disease Resistance Gene Lr34 Confers Fungal Disease Resistance in Maize
Beyond Crop Biotech
- Scientists Identify Key Catalyst for Antibiotic Resistance
- Researchers Show Parasites' Diet Can Affect DNA
- 2016 EU Agricultural Outlook Conference
- Forum of Scientific Society Leaders on Genetically Engineered Crops: Experiences and Prospects
- New ISAAA Video Presents Impact of GM Crops on Adopting and Importing Countries
- Researchers Study the Use of CRISPR/Cas9 to Generate Virus Resistance
- CRISPR/Cas9 Genome Editing of Rubber-Producing Dandelion
Read the latest:
- Biotech Updates (February 1, 2023)
- Genome Editing Supplement (January 18, 2023)
- Gene Drive Supplement (January 25, 2023)
Subscribe to BU: