Plant Defensin from Alfalfa Confers Resistance to Leaf Rust in Transgenic WheatSeptember 7, 2016
Leaf rust caused by the fungus Puccinia triticina (Pt) is an economically important wheat disease causing significant yield losses. While resistant cultivars have been used to control it, genetic resistance is brief and is ineffective with the emergence of new virulent races. Development of new transgenic wheat expressing an antifungal defensin offers a promising approach to combat the disease.
The research team led by Jagdeep Kaur from the Donald Danforth Plant Science Center transformed two wheat genotypes using a chimeric gene encoding an antifungal plant defensin, MtDEF4.2, from alfalfa (Medicago truncatula). The transgenic lines developed from independent events were then evaluated.
Analysis revealed that homozygous transgenic wheat lines expressing MtDEF4.2 displayed resistance to the Pt race MCPSS compared to non-transgenic controls. Further analysis suggested the presence of both pre- and post-haustorial resistance to leaf rust in the transgenic lines. However, MtDEF4.2 did not affect the colonization of the beneficial mycorrhizal fungus Rhizophagus irregularis in the roots.
The results suggest that the plant defensin MtDEF4.2 can provide substantial resistance to leaf rust in transgenic wheat without affecting the plants symbiosis with the beneficial mycorrhizal fungus.
For more information on this study, read the full article in Transgenic Research.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- FAO and WFP Leaders Urged by Pope Francis to Continue Efforts to Combat Hunger
- Youth Learn about Biotech at the AgriKool Expo in Uganda
- Scientists Reveal Genetic Ancestry of Cultivated Strawberry
- Improved Procedure Accelerates Tomato Engineering
- Researchers Create Plant that Grows Fast and Defends Itself from Insects
- Australian OGTR Invites Comments for Commercial Release of GM Cotton
- Biotech Mustard is Safe, Says India's Technical Committee
- Study Reports that Plants are Adapting to Increasing Atmospheric CO2
- VIB Fact Series on Bananas and GM Food Safety Released
- HT Soybean (DAS-44406-6) Grown in Brazil is Compositionally Equivalent to Non-GM Soybean
- Plant Defensin from Alfalfa Confers Resistance to Leaf Rust in Transgenic Wheat
Beyond Crop Biotech
- Australian Scientists Decode Clover DNA
- Researchers Evaluate Health of Transgenic Donor Pigs Expressing Five Human Genes
- Agrobacterium-delivered CRISPR/Cas9 for Targeted Mutagenesis in Maize
Read the latest:
- Biotech Updates (March 29, 2023)
- Genome Editing Supplement (March 22, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: