Clavamox Suitable as Antibiotic for Agrobacterium-Mediated Transformation of the Chrysanthemum ‘Vivid Scarlet'
July 30, 2014| |
Genetic transformation requires suitable antibiotics for regeneration of transformed tissues. A study conducted by Kyungpook National University led by Chang Kil Kim, examined the effects of antibiotics carbenicillin, cefotaxime, and Clavamox on in vitro plant regeneration of Chrysanthemum morifolium ‘Vivid Scarlet'.
Shoot induction and the number of shoots per explant were recorded after 5 weeks of culture. Carbenicillin and Clavamox had less inhibitory effects on number of shoots per explant than cefotaxime. Superior plant growth was observed in shoots treated with 125 mg/L Clavamox. No variation was observed in ploidy level between control plants and plants regenerated in vitro with 125 mg/L Clavamox.
These findings suggest that Clavamox can effectively replace carbenicillin or cefotaxime in Agrobacterium-mediated transformations of ‘Vivid Scarlet'.
Visit http://www.pomics.com/naing_7_4_2014_237_243.pdf for more details on this promising study.
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