Biotech Updates

Low Level Laser Therapy (LLLT) Stimulates Release of Fibroblast Growth Factors in Diabetic Mice

May 14, 2014

Low Level Laser Therapy (LLLT) has shown great potential for use in wound healing through biostimulation. Biostimulation causes the release of growth factors that facilitate wound healing and increasing new capillary formation. For this study, the goal was to determine the effect of infrared laser light on in vitro expression of Fibroblast Growth Factor (FGF), Platelet Derived Growth Factor (PDGF) and Vascular Endothelial Growth Factor (VEGF), which are important growth factors in wound healing.

Fibroblasts were extracted from the skin of 7 diabetic and 7 non-diabetic mice and were cultured. Experimental cell cultures were irradiated with single dose of LLLT using an 810 nm continuous wave laser while the control group was not irradiated. Secretion of growth factors by skin fibroblasts were quantified through real time polymerase chain reaction.

Irradiated fibroblasts from diabetic mice showed significant increase in FGF expression. However, irradiation had no significant effect on the levels of PDGF and VEGF in both diabetic and non-diabetic cultures. Based on the results, LLLT may play a promising important role in wound healing through stimulation of fibroblasts.

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