Alginate Gel-Based 3D Cell Culture Developed for Cancer Stem Cell ResearchApril 30, 2014
One of the most recent models of tumorigenesis in cancer is the cancer stem cell (CSC) model. CSCs play an important role in tumor initiation, progression and metastasis. They are also responsible for the high therapeutic failure rates against cancer. Although widely used in tumor studies, the two-dimensional (2D) cell culture has limitations in maintaining CSCs in vitro. Therefore, it is necessary to develop an efficient culture system for further research on CSCs.
The three-dimensional (3D) cell culture has the ability to better recapitulate the in vivo microenvironment than 2D cell culture and has been proven to successfully improve CSC properties. Hence, it is a leading candidate as the new culture system for CSCs. In the study, the three-dimensional (3D) culture system based on alginate gel (ALG) beads was evaluated. Two cell lines derived from different histologic origins were encapsulated in ALG beads and the expansion of CSCs was investigated.
The proportion of cells with CSC-like phenotypes increased significantly in ALG beads compared to 2D culture. CSCs cultured in ALG beads also had increased metastasis ability and greater expression levels of CSC-related genes. It was also found that hypoxia-inducible factors (HIFs) that occurred in ALG beads were responsible for inducing the increase in CSC proportion. Therefore, ALG bead was an efficient culture system for CSC enrichment, which might provide a useful platform for research and development of new anti-cancer therapies.
Read more at http://www.sciencedirect.com/science/article/pii/S0168165614000893.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Climate Change Study Says Biotech Helps Achieve Nitrogen Fertilizer Management
- Nigeria's Supervising Minister for S&T Acknowledges Need for Biosafety Law
- Kenyan Stakeholders Tell Government Taskforce to Lift GM Food Imports Ban
- Zambia Cotton Board Calls for GM Cotton
- Transgenic Sweet Potato to Battle Virus Diseases in South Africa
- CAST Releases Issue Paper on GE Food Labeling
- Initiative Creates Team of Engineers, Plant Scientists to Develop Smart Plants
- Sub-Lethal Doses of Herbicide Could Prevent Transgene Escape to Weedy Brassica Species
- Study Finds Plants Send Out Signals that Attract Harmful Bacteria
- Iran Initiates Field Trial of Insect Resistant Rice and Cotton
- Scientists Identify Molecular Transporters that Drive Plant Responses to Drought and Salinity
- Fischler: GM Soybean Vital to Egg Producers in Europe
- Tobacco Companies Turn to Biotechnology
- Scientists Seek to Fine Tune Flowering Time
- CAS Scientists Increase Essential Fatty Acid in Rice Bran
- Effects of Seed-Specific Gene Silencing of OsMRP5 Gene in Transgenic Rice
- CryF1 Proteins Has No Effects on Cotesia marginiventris
Beyond Crop Biotech
- International Team Sequences Rainbow Trout Genome
- Potential Pharmaceuticals Derived from Rice Endosperm
- Alginate Gel-Based 3D Cell Culture Developed for Cancer Stem Cell Research
Read the latest:
- Biotech Updates (May 31, 2023)
- Gene Editing Supplement (May 31, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: