Transgenic Sweet Potato to Battle Virus Diseases in South AfricaApril 30, 2014
Infection of sweet potato by multiple viruses is a common occurrence and often results as a more severe disease than the sum effect of infection with each virus alone. The sweet potato feathery mottle virus (SPFMV), sweet potato chlorotic stunt virus (SPCSV), sweet potato virus G (SPVG) and Sweet potato mild mottle virus (SPMMV) have caused a synergistic disease complex that has devastated sweet potato in South Africa. In order to address the problem, transgenic sweet potato (Ipomoea batatas Lam.) with broad virus resistance is being developed through genetic engineering.
Transformation of apical tips of sweet potato cultivar Blesbok was achieved through Agrobacterium tumefaciens strain LBA4404. Coat protein gene segments of the four viruses were used to induce gene silencing in transgenic sweet potato. Six transgenic plants were developed and these six were then evaluated through graft inoculation with SPFMV, SPCSV, SPVG and SPMMV-infected Ipomoea setosa. Virus presence was detected in the transgenic sweet potatoes using nitrocellulose enzyme-linked immunosorbent assay. However, all transgenic plants displayed delayed and milder symptoms of chlorosis and mottling of lower leaves compared to the control plants.
These results showed promise and require further investigation on resistance to virus infections under field conditions.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Climate Change Study Says Biotech Helps Achieve Nitrogen Fertilizer Management
- Nigeria's Supervising Minister for S&T Acknowledges Need for Biosafety Law
- Kenyan Stakeholders Tell Government Taskforce to Lift GM Food Imports Ban
- Zambia Cotton Board Calls for GM Cotton
- Transgenic Sweet Potato to Battle Virus Diseases in South Africa
- CAST Releases Issue Paper on GE Food Labeling
- Initiative Creates Team of Engineers, Plant Scientists to Develop Smart Plants
- Sub-Lethal Doses of Herbicide Could Prevent Transgene Escape to Weedy Brassica Species
- Study Finds Plants Send Out Signals that Attract Harmful Bacteria
- Iran Initiates Field Trial of Insect Resistant Rice and Cotton
- Scientists Identify Molecular Transporters that Drive Plant Responses to Drought and Salinity
- Fischler: GM Soybean Vital to Egg Producers in Europe
- Tobacco Companies Turn to Biotechnology
- Scientists Seek to Fine Tune Flowering Time
- CAS Scientists Increase Essential Fatty Acid in Rice Bran
- Effects of Seed-Specific Gene Silencing of OsMRP5 Gene in Transgenic Rice
- CryF1 Proteins Has No Effects on Cotesia marginiventris
Beyond Crop Biotech
- International Team Sequences Rainbow Trout Genome
- Potential Pharmaceuticals Derived from Rice Endosperm
- Alginate Gel-Based 3D Cell Culture Developed for Cancer Stem Cell Research
Read the latest:
- Biotech Updates (November 29, 2023)
- Gene Editing Supplement (November 29, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: