Species: Goat (Capra hircus)

Country of 1st Author: China
Year: 2014

Institute	College of Life Sciences, Shihezi University Shihezi, Xinjiang CN
Editing Method	Cloned cell line
SDN Type	SDN-1
Delivery	Cells were transfected with a plasmid (2 mg) expressing both Cas9 and gRNA targeting MSTN1 (single targeting), two plasmids (2 mg of each plasmids) expressing Cas9 and gRNA targeting MSTN-1 and PrP genes (double targeting), or four plasmids (2 mg of each plasmids) expressing Cas9 and gRNA targeting MSTN-1, PrP, BLG and NUP-1 genes (quadruple targeting) using Nucleofector
Remarks	A single co-transfection of pooled Cas9/gRNAs enabled isolation of cell colonies carrying simultaneous disruption of four genes with high efficiency.
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
MSTN Myostatin	Double muscle	Yield	CRISPR Cas9 (spCas9)
NUP155 nucleoporin 155		Yield	CRISPR Cas9 (spCas9)
PrP prion protein		Yield	CRISPR Cas9 (spCas9)
BLG β-Lactoglobulin	Knockout of beta-lactoglobulin for hypoallergenic milk	Hypoallergenic	CRISPR Cas9 (spCas9)

References, Documents, and Links

• Efficient Gene Knockout in Goats Using CRISPR/Cas9 System