Species: Cattle (Bos taurus)

Country of 1st Author: China
Year: 2021

Institute	China Agricultural University Beijing, 100193 CN
Editing Method	Cloned cell line
SDN Type	SDN-3
Delivery	The gene-targeting vector pSRY-EGFP was constructed as shown in Fig. 2a. We introduced Sry TALEN pair 1, along with a 9 kb dsDNA donor construct pSRY-EGFP that contained the self-cleaving peptide the 2A regions of Thosea asigna virus (T2A) incorporated EGFP (T2A-EGFP) and a positive selection marker Neomycin (NEO) flanked by short homologous arms (843 bp and 910 bp for 5' and 3' arms, respectively) and a negative selection marker diphtheria toxin A (DTA)
Foreign DNA	Yes
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
SRY Sex Determining Region Y	Sex reversal of XY males into females	Reproduction	TALEN
ZFY Zinc Finger Protein Y-Linked	Sex reversal in XY to be female	Reproduction	TALEN
DDX3Y DEAD-Box Helicase 3 Y-Linked	Sex reversal in XY to be female	Reproduction	TALEN
Eif2s3y eukaryotic translation initiation factor 2, subunit 3, structural gene Y-linked	Sex reversal in XY to be female	Reproduction	TALEN

References, Documents, and Links

• Efficient TALEN-mediated gene knockin at the bovine Y chromosome and generation of a sex-reversal bovine