Species: Cattle (Bos taurus)

Country of 1st Author: China
Year: 2021

Institute	Northwest A&F University Yangling 712100, Shaanxi CN
Editing Method	Cloned cell line
SDN Type	SDN-2
Delivery	Using the HMEJ-based method, we targeted to the bROSA26 locus to stimulate functional NRAMP1 gene KI and ultimately more effectively produced gene-edited cattle.
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
NRAMP1 Natural resistance-associated macrophage protein-1	Resistance to tuberculosis	Biotic Stress	CRISPR Cas9 (spCas9)	Plasmids encoding Cas9 protein, Cas9/sgRNA11, were cotransfected with pROSA26-SA-EGFP-Puro-HDR into BFFs to achieve stable genetic modification of cells that were targeted to bROSA26 locus through HDR - the integration was of BOVINE NRAMP1

References, Documents, and Links

• HMEJ-based safe-harbor genome editing enables efficient generation of cattle with increased resistance to tuberculosis