Species: Cattle (Bos taurus)

Country of 1st Author: South Korea
Year: 2022

Institute	Seoul National University, Gwanak-gu 151-921 KR
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	exon 2 targeted and sgRNA, Cas0 mRNA and sgRNA were injected into zygotes
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
MSTN Myostatin	Double muscle	Yield	CRISPR Cas9 (spCas9)	exon 2 targeted and sgRNA, Cas0 mRNA and sgRNA were injected into zygotes

References, Documents, and Links

• Production of MSTN-mutated cattle without exogenous gene integration using CRISPR-Cas9